Expression of a sphingomyelinase coding gene from Trichoderma harzianum conferred bacterial tolerance in tobacco

Abstract Diseases caused by bacteria are an important and widespread constraint, occurring in almost all crops, including vegetables, pulses, cereals, ornamentals, fruit forages. Although several strategies have been developed to obtain disease resistance plants using genetic engineering, few studies effectively demonstrated the control of bacterial diseases. It has previously reported that a gene encoding sphingomyelinase ( ThSMase ), identified Trichoderma harzianum , is up‐regulated during biocontrol against phytopathogens, this may biotechnological applications. SMases involved multiple cellular functions, immune response pathogens. We hypothesized expression from fungi transgenic tobacco could generate plant was cloned under double CaMV 35S promoter plus leader sequence alfalfa mosaic virus (AMV), stably introduced expressed tobacco. Reverse transcription‐quantitative PCR analysis revealed at similar levels lines tested. Our results showed no statistically significant difference susceptibility after challenging nontransgenic with fungus Sclerotinia sclerotiorum . However, bacterium Pseudomonas syringae pv. tabaci tolerance Xylella fastidiosa strong potential processes such as molecular breeding plants.